Foodomics
Laboratory
경희대학교 식품오믹스 연구실
Research
멀티오믹스 기반 발효식품 평가 및 분석
본 연구실은 발효식품의 복합적인 미생물 생태계와 대사 과정을 다각적으로 분석하기 위해 멀티 오믹스(Multi-Omics) 접근법을 활용하고 있습니다. 이를 통해 발효 과정에서 미생물 군집의 변화와 주요 대사 경로를 규명하고, 생성되는 대사산물과 단백질을 분석하여 기능성 성분의 변화를 추적하며, 유해 미생물의 증식 여부 및 유전자 발현 패턴을 분석하여 안전성을 확보합니다. 또한, 전통 발효식품의 과학적 근거를 제공하고 현대적 식품 가공기술과 융합하여 새로운 기능성 발효식품을 개발하는 데 집중하고 있습니다. 이를 위해 차세대 염기서열 분석(NGS, Next-Generation Sequencing), 메타볼로믹스(Metabolomics), 단백질체학(Proteomics), 생물정보학 분석, 기능성 평가 등의 다양한 방법을 활용하며, 발효식품의 품질 개선과 기능성 증대를 위한 혁신적인 접근법을 모색하고 있습니다.
식품 부산물의 업사이클링을 통한 고부가가치화 연구
식품 산업에서 발생하는 다양한 부산물을 유산균 발효 기반 Bioconversion 기술을 활용하여 신규 기능성 식품 소재로 업사이클링하는 연구를 수행하고 있습니다. 이를 위해 부산물 발효의 최적 조건을 확립하고, 질량분석 기반 대사산물 분석을 통해 발효 전후의 성분 변화를 조사하며, 유용 물질을 탐색하고 있습니다. 또한, exopolysaccharide(EPS), 프리바이오틱스 등 고부가가치 소재를 추출하여 다양한 기능성을 평가하고, 발효 균주의 프로바이오틱스 특성을 검증하기 위해 in vitro 및 in silico 기반의 다양한 분석 방법을 활용하여 신규 프로바이오틱스를 개발하고 있습니다. 이를 통해 유산균의 역할과 기능성 향상의 기작을 규명하고, 신규 소재에 대한 이해를 확장하여 친환경적이며 지속 가능한 식품 개발을 위한 과학적 기반을 마련하고 있습니다.
식품 안전성 평가를 위한 검출법 개발 및 신규 소재 검증
식품 안전성 평가를 위한 정밀한 검출법을 개발하고, 신규 식품 소재의 안전성을 검증하는 연구를 수행하고 있습니다. 이를 위해 LAMP, real-time PCR, digital PCR과 같은 다양한 분자 생물학적 분석 기법을 활용하여 식품 원료의 유전체 정보를 정밀하게 분석하고, 특정 타겟에 대한 고유한 분자 마커를 발굴하는 연구를 진행하고 있으며, 신속 정확한 검출법 개발, 식품의 위해요소 확인, 식품의 위·변조 방지 및 소비자 안전 확보에 기여하고 있습니다. 또한, 식용 곤충, 열대 과일과 같은 신규 식품 소재의 안전성을 검증하기 위해, 식품 소재간의 알레르기 교차반응을 확인하고 신규 소재 검출법을 개발하는 연구를 진행하고 있습니다. 이를 통해 신규 식품 원료의 안전성 평가 기준을 마련하고, 식품 산업의 신뢰도를 높일 수 있는 객관적 평가 체계를 구축하고 있습니다.
Publication
Development and validation of a multiplex real-time PCR assay for accurate authentication of common buckwheat (Fagopyrum esculentum) and tartary buckwheat (Fagopyrum tataricum) in food. Food Control, 145, 109442.
Duplex nanoplate-based digital PCR assay for simultaneous detection of pork and chicken in processed meat products. Journal of Food Composition and Analysis, 140, 107246.
Effects of white-colony forming yeast (WCFY) on microbial communities and metabolome in Kimchi. Food Chemistry, 465, 142059.
2025
2024
A Small Epitope Tagging on the C-Terminus of a Target Protein Requires Extra Amino Acids to Enhance the Immune Responses of the Corresponding Antibody, In: Journal of Microbiology and Biotechnology. 34, 6, p. 1222-1228 7 p.
A study at the wildlife-livestock interface unveils the potential of feral swine as a reservoir for extended-spectrum β- lactamase-producing Escherichia coli, In: Journal of Hazardous Materials. 473, 134694.
Effects of storage temperature on the diversity of white colony-forming yeast and correlations between bacterial and yeast communities in salted kimchi cabbage, (Accepted/In press) In: Food Science and Biotechnology.
Human Milk-Derived Enterococcus faecalis HM20: A Potential Alternative Agent of Antimicrobial Effect against Methicillin- Resistant Staphylococcus aureus (MRSA), In: Microorganisms. 12, 2, 306.
Loop-mediated isothermal amplification (LAMP) assays for rapid on-site detection of Lacticaseibacillus casei, Lacticaseibacillus paracasei, and Lacticaseibacillus rhamnosus in probiotic products, In: Food Bioscience. 57, 103562.
Rapid on-site detection of Leuconostoc citreum in commercially processed products using loop-mediated isothermal amplification(LAMP) technique, In: Food Control. 158, 110230.
2023
Development of real-time PCR method for rapid and accurate detection of Centipedes (Scolopendra mutilans) in food, In: Food Science and Biotechnology. 32, 7, p. 979-985 7 p.
Novel Heptaplex PCR-Based Diagnostics for Enteric Fever Caused by Typhoidal Salmonella Serovars and Its Applicability in Clinical Blood Culture, In: Journal of Microbiology and Biotechnology. 33, 11, p. 1457-1466 10 p.
2022
A novel combination therapy for multidrug resistant pathogens using chitosan nanoparticles loaded with β-lactam antibiotics and β-lactamase inhibitors, In: International Journal of Biological Macromolecules. 195, p. 506-514 9 p.
Comparison of fermentation characteristics of kimchi made with fresh and stored spring kimchi cabbage, In: Food Science and Biotechnology. 31, 2, p. 221-229 9 p.
Comprehensive in vitro and in vivo risk assessments of β-lactam antibiotic and β-lactamase inhibitor loaded chitosan nanoparticles, In: Journal of Industrial and Engineering Chemistry. 113, p. 181-188 8 p.
Development of ultrafast PCR assays for the event-specific detection of eleven approved genetically modified canola events in South Korea, In: Food Chemistry. 373, 131419.
2021
Detection of GM Canola MS11, DP-073496-4, and MON88302 events using multiplex PCR coupled with capillary electrophoresis, In: Food Science and Biotechnology. 30, 4, p. 565-570 6 p.
Development of triplex PCR for simultaneous detection of soybean and wheat, In: Food Science and Biotechnology. 30, 6, p. 801-806 6 p.
Development of ultrafast PCR for rapid detection of buckwheat allergen DNA (fag e 1) in processed foods, In: Food Control. 130, 108334.
Direct loop-mediated isothermal amplification (LAMP) assay for rapid on-site detection of Bifidobacterium longum subspecies longum, infantis, and suis in probiotic products, In: Food Chemistry. 346, 128887.
Direct triplex loop-mediated isothermal amplification assay for the point-of-care molecular detection of Salmonella genus, subspecies I, and serovar Typhimurium, In: Food Control. 120, 107504.
Growth inhibitory effect of garlic powder and cinnamon extract on white colony-forming yeast in Kimchi, In: Foods. 10, 3, 645.
Novel approaches for the identification of microbial communities in kimchi: MALDI-TOF MS analysis and high-throughput sequencing, In: Food Microbiology. 94, 103641.
Physicochemical Characteristics and Microbial Communities in Gochujang, a Traditional Korean Fermented Hot Pepper Paste, In: Frontiers in Microbiology. 11, 620478.
Real-time PCR assays for the quantitative detection of Kazachstania servazzii and Candida sake related to undesirable white colony on kimchi, In: Food Control. 125, 107984.
The Gut Microbiota of Newborn Calves and Influence of Potential Probiotics on Reducing Diarrheic Disease by Inhibition of Pathogen Colonization, In: Frontiers in Microbiology. 12, 772863.
2020
A multiplex PCR assay combined with capillary electrophoresis for the simultaneous detection of tropomyosin allergens from oyster, mussel, abalone, and clam mollusk species, In: Food Chemistry. 317, 126451.
Development of a real-time PCR assay for the detection of donkey (Equus asinus) meat in meat mixtures treated under different processing conditions, In: Foods. 9, 2, 130.
Development of Real-Time PCR Assay to Specifically Detect 22 Bifidobacterium Species and Subspecies Using Comparative Genomics, In: Frontiers in Microbiology. 11, 2087.
Impact of fermentation conditions on the diversity of white colony-forming yeast and analysis of metabolite changes by white colony-forming yeast in kimchi, In: Food Research International. 136, 109315.
Species identification of red deer (Cervus elaphus), roe deer (Capreolus capreolus), and water deer (Hydropotes inermis) using capillary electrophoresis-based multiplex PCR, In: Foods. 9, 8, 0982.
2019
A fast multiplex real-time PCR assay for simultaneous detection of pork, chicken, and beef in commercial processed meat products, In: LWT - Food Science and Technology. 114, 108390.
Development and validation of ultrafast PCR assays to detect six species of edible insects, In: Foods. 9, 2, 130.
Development of Real-Time PCR Assay to Specifically Detect 22 Bifidobacterium Species and Subspecies Using Comparative Genomics, In: Food Control. 103, p. 21-26 6 p.
Development of a direct loop-mediated isothermal amplification (LAMP) assay for rapid and simple on-site detection of chicken in processed meat products, In: Food Control. 98, p. 194-199 6 p.
Development of a fast real-time PCR assay based on TaqMan probe for identification of edible rice grasshopper (Oxya chinensis) in processed food products, In: Food Research International. 116, p. 441-446 6 p.
Mixed starter of Lactococcus lactis and Leuconostoc citreum for extending kimchi shelf-life, In: Journal of Microbiology. 57, 6, p. 479-484 6 p.
Rapid on-site detection of shrimp allergen tropomyosin using a novel ultrafast PCR system, In: Food Science and Biotechnology. 28, 2, p. 591-597 7 p.
Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR, In: Food Science and Biotechnology. 28, 5, p. 1593-1598 6 p.
2018
Comparison of methods of DNA extraction from tree nuts, In: Korean Journal of Food Science and Technology. 50, 4, p. 357-361 5 p.
Development and validation of a multiplex PCR assay for simultaneous detection of chicken, turkey and duck in processed meat products, In: International Journal of Food Science and Technology. 53, 12, p. 2673-2679 7 p.
Development of a direct loop-mediated isothermal amplification (LAMP) assay for rapid and simple on-site detection of chicken in processed meat products, In: Food Control. 98, p. 194-199 6 p.
Development of a fast duplex real-time PCR assay for simultaneous detection of chicken and pigeon in raw and heat-treated meats, In: Food Control. 85, p. 1-5 5 p.
Development of detection method for edible silkworm (Bombyx mori) using real-time PCR, In: Food Control. 94, p. 295-299 5 p.
Direct duplex real-time loop mediated isothermal amplification assay for the simultaneous detection of cow and goat species origin of milk and yogurt products for field use, In: Food Chemistry. 246, p. 26-31 6 p.
Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR, In: Journal of Microbiology and Biotechnology. 28, 2, p. 210-217 8 p.
2017
Draft genome sequence of pediococcus pentosaceus strain FBL2, a probiotic bacterium isolated from jogaejeot, a salted fermented food, in the Republic of Korea, In: Genome Announcements. 5, 18, e00303-17.
Rapid identification of vibrio species isolated from the southern coastal regions of Korea by MALDI-TOF mass spectrometry and comparison of Maldi sample preparation methods, In: Journal of Microbiology and Biotechnology. 27, 9, p. 1593-1601 9 p.
Species identification of commercial jerky products in food and feed using direct pentaplex PCR assay, In: Food Control. 78, p. 1-6 6 p.
2016
Development of a one-step duplex RT-PCR method for the simultaneous detection of VP3/VP1 and VP1/P2B regions of the hepatitis a virus, In: Journal of Microbiology and Biotechnology. 26, 8, p. 1398-1403 6 p.
Development of a rapid on-site detection method for pork in processed meat products using real-time loop-mediated isothermal amplification, In: Food Control. 66, p. 53-61 9 p.
Quantitative detection of pork in commercial meat products by TaqMan® real-time PCR assay targeting the mitochondrial D-loop region, In: Food Chemistry. 210, p. 102-106 5 p.
2015
Development of duplex PCR method for simultaneous detection of rabbit (Oryctolagus cuniculus) and cat (felis catus) meats, In: Journal of Applied Biological Chemistry. 58, 4, p. 383-387 5 p.
Multiplex PCR for simultaneous identification of turkey, ostrich, chicken, and duck, In: Journal of the Korean Society for Applied Biological Chemistry. 58, 6, p. 887-893 7 p.
2014
Characterisation of inorganic elements and volatile organic compounds in the dried sea cucumber Stichopus japonicus, In: Food Chemistry. 147, p. 34-41 8 p.
Identification of Lactic Acid Bacteria in Pork Meat and Pork Meat Products Using SDS-PAGE, 16S rRNA Gene Sequencing and MALDI-TOF Mass Spectrometry, In: Journal of Food Safety. 34, 3, p. 224-232 9 p.